Basic Biochemical Laboratory Procedures and Computing: With Principles, Review Questions, Worked Examples and Spreadsheet Solutions

Provides theoretical background for some widely used biomolecular calculations and several modern research procedures. It also provides worked examples and spreadsheet templates of numerous biomolecular calculations. The approach taken is multidisciplinary. Thus, some topics discussed may be found in textbooks of biology, general chemistry, statistics, or computer science. Other topics discussed may be found in the primary biomedical research journals, annual review publcations and momgraphs. Whenever possible, applications of the topics discussed to recent research in clinical, academic, industrial,and basic research laboratories are pointed out explicitly. Thus Basic Biomedical Laboratory Procedures and Computing provides theoretical background and spreadsheet solutions in selected areas of the contemporary biomedical sciences; It also feflects the thinking, methods, instruments and experimental strategies of today's practising biomedical scientists.
Contents

PART I. BIOANALYTICAL CHEMISTRY
1. Solutions: Preparation and concentration
2. Acids and bases
3. Countercurrent distribution and chromatography
PART II. MACROMOLECULAR STRUCTURE AND ASSAY
4. The separation and characterization of macromolecules
5. Kinetics and binding equilibria
6. Molecular immunology and immunochemical assays
PART III. PHYSIOCHEMICAL APPROACHES
7. Biomolecular spectroscopy
8. Radioactivity
9. Oxidation reduction
PART IV. DATA ANALYSIS
10. One- and Two-sample analyses
11. Analysis of variance
Appendices