Handbook of Molecular and Cellular Methods in Biology and Medicine

rovides an updated took kit that incorporates conventional and modern approaches
Presents Troubleshooting Guides in most chapters indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures
Includes diagrams, tables, and figures to clarify methods and results expected from their use
Contains the most relevant lab protocols in use today

Summary

Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era.

Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA ? RNA ? protein ? metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy.

In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.

Table of Contents

DNA-Based Technology
Isolation and Purification of DNA; L. J. Cseke, C. An, and C.-J. Tsai
DNA Cloning Strategies; L. J. Cseke, G. Trivedi, A. Sreedasyam, P. B. Kaufman, and A. Kirakosyan
Polymerase Chain Reaction Methodologies; A. Kirakosyan, P. B. Kaufman, L. J. Cseke, and E. M. Seymour
Preparation of Nucleic Acid Probes; L. J. Cseke and L. R. Cruz-Vera
Southern Blot Hybridization; C.-J. Tsai and L. J. Cseke
Genomic DNA Libraries; L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
DNA Sequencing and Analysis; L. J. Cseke and L. Larka
RNA-Based Technology
Isolation and Purification of RNA; C.-Jui Tsai, L. J. Cseke, and S. A. Harding
RT-PCR and qRT-PCR Methodologies; A. K. Pandey, L. J. Cseke, M. R. Davis, P. B. Kaufman, E. M. Seymour, and A. Kirakosyan
Preparation of Nucleic Acid Probes; A. Kirakosyan, P. B. Kaufman, and L. J. Cseke
Northern Blot Hybridization; A. Kirakosyan, P. B. Kaufman, C.-J. Tsai, and L. J. Cseke
cDNA Libraries; L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
Differential Display; Y.-S. Wang, S. A. Harding, and C.-Jui Tsai
Localization of RNA Expression; S. A. Harding, C.-Jui Tsai, and L. J. Cseke
Isolation and Purification of Proteins; L. J. Cseke, P. B. Kaufman, A. Kirakosyan, and M. V. Westfall
Western Blot Hybridization; P. B. Kaufman, A. Kirakosyan
and E. M. Seymour
Localization of Protein Expression; A. Kirakosyan, P. B. Kaufman, M. V. Westfall, M. Mashore, C. R. Lu, and E. M. Seymour
DNA Footprinting and Gel Retardation Assay; L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
Protein–Protein and Protein–Ligand Interactions; M. D. Yoder
Metabolites
Isolation and Purification of Metabolites; A. Kirakosyan, K. R. Noon, M. McKenzie, L. J. Cseke, and P. B. Kaufman
Analysis of Metabolites; K. R. Noon, A. Kirakosyan, M. McKenzie,
and P. B. Kaufman
Genomics, Proteomics, and Metabolomics
Bioinformatics and Data Analysis; S. Holmes and O. De la Cruz Cabrera
Microarray Platforms; K. P. Venkataramanan, L. J. Cseke, Gopi K. Podila, A. Kirakosyan, and P. B. Kaufman
Microarray Data Collection; K. P. Venkataramanan, L. J. Cseke, G. K. Podila,
A. Kirakosyan, and P. B. Kaufman
Microarray Data Analysis; K. P. Venkataramanan, L. J. Cseke, G. K. Podila, P. B. Kaufman, and A. Kirakosyan
Proteomics: Data Collection and Analysis; E. H. Kim, D. E. Misek, and M. V. Westfall
Metabolomics Data Collection and Analysis; T. Okada and A. Katoh
Common Protocols on Membrane Lipid Analysis and Lipidomics; A. K. Das
Integrated Functional Genomics; L. J. Cseke, F. Chen, P. B. Kaufman, and A. Kirakosyan
Manipulation of Biological Systems
Inhibition of Gene Expression; J. M. Agee, L. Boyd, and L. J. Cseke
Inhibition of Protein Synthesis; P. B. Kaufman, A. Kirakosyan, and L. J. Cseke
Gene Transfer and Expression in Animal Cells; S. E. Kampert, E. Devaney, and M. V. Westfall
Methods of Stem Cell Lineage Analysis In Situ and in Cell Culture; A. B. Borisov
Tissue Engineering of Fully Differentiated Tissue: Lessons from Work on Cardiac Myocytes; T. K. Stevenson and M. V. Westfall
Plant Tissue and Cell Culture; N. P. Chanana, L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
Gene Transfer and Expression in Plants; R. C. Thakur, L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
Macromolecular Analyses
Microscopy: Light and Confocal; C. R. Lu, P. B. Kaufman, and L. J. Cseke
Microscopy: Scanning Electron, Environmental Scanning Electron, and Transmission Electron; C. R. Lu, P. B. Kaufman, A. Kirakosyan, and L. J. Cseke
Laser Capture Microdissection and Whole Genome Amplification; R. Buyyarapu, V. R. Sripathi, S. B, Cseke, R. V. Kantety
Index