Textbook of Assisted Reproductive Techniques Laboratory and Clinical Perspectives

'This thoughtfully assembled book will guide, assist, and provide foundation and reference to both the neophyte and the seasoned ARTisan.' - Fertility and Sterility (of the first edition)

'The book's real value is that we discuss a day-to-day problem in the unit and know immediately where to look'. - orgyn.com (of the first edition)

'If ART is your field, then anything you may want to look up or simply browse through, you will find in this very comprehensive and competent volume.' - ObGyn.net (of the first edition)

A truly comprehensive manual for the whole team at the IVF clinic. Information is presented in a highly visual manner, allowing both methods and protocols to be consulted easily. The second edition of this extremely popular textbook has been revised throughout and includes new chapters on quality control and regulatory control.

Contents:
LABORATORY PROCEDURES
Section I: Setting Up an IVF Laboratory
1. Setting Up an ART Laboratory 2. Quality Control: Maintaining Stability in the Laboratory 3. Accreditation of the ART LAboratory: the North American Perspective 4. Accreditation of the ART Laboratory: the European Perspective
Section II: Handling and Preparation of the Sperm and Oocytes
5. Evaluation of Sperm 6. Sperm Preparation Techniques 7. Sperm Chromatin Assessment 8. Oocyte Treatment: from Retrieval to Insemination 9. Preparation and Evaluation of Oocytes for Intracytoplasmic Sperm Injection 10. Oocyte In-vitro Maturation
Section III: Micromanipulation
11. Equipment and General Technical Aspects for Micromanipulation of Gametes and Embryos 12. Intracytoplasmic Sperm Injection: Technical Aspects 13. Assisted Hatching 14. Human Embryo Biopsy for Preimplantation Genetic Diagnosis
Section IV: Culture of the Embryo and Embryo Transfer
15. Analysis of Fertilization 16. Culture Systems for the Human Embryo 17. Evaluation of Embryo Quality: Sequential Analysis of Embryo Development with the Aim of Single Embryo Transfer
Section V. Cryopreservation of Gametes and Embryos
18. Slow Freezing of Human Oocytes 19. Vitrification of Human Oocytes 20. Slow Freezing of Human Embryos 21. Vitrification of Human Embryos 22. Managing the Cryopreserved Embryo Bank 23. Cryopreservation and Storage of Spermatozoa 24. Handling and Cryopreservation of Testicular Sperm 25. Ovarian Tissue Cryopreservation and Other Fertility Preservation Strategies
Section VI: Diagnosis of Genetic Disease in Preimplantation Embryos
26. Preimplantation Genetic Diagnosis Using Comparative Genomic Hybridization 27. Severe Male Factor: Genetic Consequences and Recommendations for Genetic Testing 28. Chromosomal Abnormalities in Human Embryos 29. Genetic Analysis of the Embryo 30. Polar Body Biopsy 31. Clinical Application of Polar Body Biopsy
Section VII: Implantation
32. Embryonic Regulation in the Implantation Process 33. The Use of Biomarkers for the Assessment of Uterine Receptivity
Section VIII: Data Management and Interpretation
34. Setting Up an ART National Registry 35. Evidence-based Medicine: Issues in Evaluating Efficacy of Treatment in Assisted Reproduction 36. Health Economics of ART

CLINICAL TECHNIQUES
Section IX: Quality Management Systems
37. Quality Management in Reproductive Medicine
Section X: Patient Investigation and the Use of Drugs
38. Indications for IVF Treatment: from Diagnosis to Prognosis 39. Initial Investigation of the Patient (Female and Male) 40. Drugs Use for Controlled Ovarian Stimulation: Clomiphene Citrate, Aromatase Inhibitors, Gonadotropins, and Gonadrotopin-releasing Hormaone Analogs 41. The Role of FSH and LH in Ovulation Induction: Current Concepts and the Contribution of Recombinant Gonadotropins 42. Developing New Therapeutics for ART: Recombinant DNA Technology and Beyond
Section XI: Stimulation Protocols
43. Endocrine Characteristics of ART Cycles 44. The Use of GnRH Agonists 45. GnRH Antagonists 46. Monitoring IVF Cycles 47. Follicle Aspiration 48. The Luteal Phase: Luteal Support Protocols 49. Evaluation and Treatment of the Low Responder Patient 50. Repeated Implantation Failure: the Prefered Therapeutic Approach
Section XII: Different Technical Procedures
51. Ultrasound in ART 52. Sperm-recovery Techniques: Clinical Aspects 53. Gamete Intrafallopian Transfer (GIFT) 54. Zygote Intrafallopian Transfer (ZIFT) 55. Embryo Transfer 56. Anesthesia and In-vitro Fertilization
Section XIII: Special Medical Conditions
57. Endometriosis and ART 58. Polycystic Ovaries and ART 59. Prognostic Tests of Ovarian Reserve 60. Management of Hydrosalpinx
Section XIV: Complications of Treatment
61. Severe Ovarian Hyperstimulation Syndrome 62. Bleeding, Severe Pelvic Infection, and Ectopic Pregnancy 63. Iatrogenic Multiple Pregnancy: the Risk of ART 64. Reducing the Incidence of Multiple Gestation
Section XV: Egg Donation and Surrogate Motherhood
65. Egg and Embryo Donation 66. Gestational Surrogacy
Section XVI: Future Directions and Clinical Applications
67. Human Embryonic Stem Cells 68. Follicle Culture
Section XVII:The Support Team
69. The Evolving Role of the ART Nurse: a Contemporary Review 70. Patient Support in the ART Program 71. The Relationship Between Stress and ART Outcome
Section XVIII: Ethics and Legislation
72. Worldwide Legislation 73. More Modern Ethical Dilemmas in ART